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Description
Human SFRP-2 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Secret Frizzled Related Protein 2 (SFRP-2) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of Secret Frizzled Related Protein 2 (SFRP-2) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Secreted Frizzled Related Protein 2 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Secreted frizzled-related protein 2 (sFRP-2) is a protein encoded by the SFRP2 gene in humans. This gene encodes members of the SFRP family that contain a cysteine-rich domain with homology to the putative Wnt-binding site of the Frizzled protein. SFRPs act as soluble regulators of Wnt signaling. Methylation of this gene is a potential marker for the presence of colorectal cancer. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenates and other biological fluids |
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4.4 ★★★★★
Based on 13 reviews
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Product Reviews
★★★★★ 5
Beneath the perfect surface
Format: Kindle
Yesteryear completely caught me off guard in the best possible way. What begins as a fascinating look into social media influence, curated perfection, and historical living slowly unfolds into something far deeper and far more emotional than I expected.
The storytelling was incredibly well done, especially the way the author balanced the polished modern influencer world against the harsh realities of 1800s frontier life. The transitions between timelines and perspectives were seamless, and by the end, every piece fit together in a way that completely redefined the story.
What made this especially compelling for me was how layered Natalie’s character felt. Her upbringing, family expectations, faith, public image, and the pressure to maintain perfection all shaped the choices she made throughout the story. Rather than feeling one-dimensional, she felt like someone slowly buckling under the weight of everything she believed she was supposed to be.
The emotional impact of this book surprised me. Beneath the historical elements and social media commentary is a story about identity, appearances, family, and the toll that constant performance can take on a person and those around them.
This is one of those books where the less you know going in, the better the experience will be. I expected an entertaining premise, but I ended up with a story that lingered long after I finished the final page.
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Reviewed in the United States on May 28, 2026
★★★★★ 3
About that twist…
Format: Kindle
SPOILER ALERT!
The thing about big, improbable twists in stories is that the less time you have to think about them, the better their effect. For fans of the classic TV show The Twilight Zone, it has always been clear that the half-hour shows were far better and far more punchy and memorable than their rather sad hour-long cousins. And a book has far, far more time to contemplate a twist than a TV show.
Unfortunately, despite some pointed observations by the author (narcissistic people are pretty much unlikable, cruelty and brutality give power to weak men, abused children very often cling to their abusers), the big, improbable twist in Yesteryear almost completely sinks the story.
The twist is the same one that sank M. Night Shyamalan’s 2004 movie The Village, and it fares no better here. Yes, people can and do live off the grid. But avoiding every single sign of civilization for years on end? Even if you’re not in a commercial flight path, there are such things as helicopters and small private planes, especially in remote areas. Perhaps people rarely stray onto private land in the wilderness, but once in a while, stray they do. And when that wilderness home was once widely publicized? Excuse me, but people are going to look for it.
This is all not to say that Yesteryear was not entertaining - it was. I read it in one sitting. The characters, as unlikable and unreliable as they are, were well drawn. A couple of the children were also quite believable, but the author’s excuse for the rest of the kids being cyphers was that their mother saw them as cyphers as well. Okay, that’s fair, but knowing them better would have enhanced the story for the reader.
This is certainly a promising book. It held my attention and was very well-written. But that twist - well, it sank M. Night Shyamalan, too.
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Reviewed in the United States on April 10, 2026
★★★★★ 4
Good read
Format: Hardcover
This book has been getting a lot of hype, and I can understand why. First, it is a very unique storyline. Very different plot. Second, the main character, Natalie, is totally unlikable. In fact, I would honestly say I loathed her. She has no likeable qualities whatsoever. I guess that is what makes you want to keep reading, to find out what happens to this nasty woman. That said, I admit I was confused at the end. I am still not sure what exactly happened to Natalie, how the situation came about, and how 10 years went by when Natalie seemed to only be telling a few months time. Was she delusional? Did she have a mental breakdown? I wish it was more clear as to what exactly happened.
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Reviewed in the United States on April 23, 2026
★★★★★ 5
What did i spend so much time reading??
Format: Kindle
Spoilers ahead This book makes me angry. A misogynistic fable that holds women as mothers as inherently either deeply flawed or as if some fairy tale perfect mother exists. I found the early parts of the novel quite funny. Thinking this poor woman why do people expect such crazy standards? Also social media influencers somehow we are to take for reality? It is entertaining and that's why we want to spend our time looking at it. It is a fantasy world. Yet that is somehow turned from something comical and poignant dissolving into madness where there can be a 16 year old that can take away your children for what I'm not sure. Filming them? Having nannies? And then again the next bunch for living off the grid? I get that this isn't going for realism. It ultimately became for me this poorly written satire maybe? Obviously what started as comically intriguing for me descended into one totally crazy crazy turn to the darkest of places. I recognize this was supposed to be some sort of cracked fairytale where nearly everyone is evil and/or mental but 30 years for child abuse? This is like a nightmare not a thriller. The book demonstrates a hatred for mothers. Just awful! It made me crave for a totally unrealistic thriller. I came to be entertained and left pissed off.
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Reviewed in the United States on May 13, 2026
★★★★★ 5
Excellent satire of tradition cult
Format: Kindle
I read this book in two days. I was eager to read it once I read the description in a Facebook post. I am a theologically conservative Christian frustrated with the tradition culture that has co-opted the faith. I see this in my social and social media circles quite a bit, and I was intrigued by a novel that took this movement to task.
Furthermore, I truly enjoy well done satire and farce. I have had a hard time finding these works written well in the past, so I was bracing myself to be disappointed. I am thankful to say, I wasn’t. I didn’t expect the novel to go the way it did (and no, I won’t post spoilers), but I literally laughed out loud a several points.
Let me compliment the author for one particular point: She doesn’t just blast the “tradwife” movement. Plenty of cultural movements and groups of people have their sins exploited. Rather than shooting a 12-gauge at religious conservatives, she takes a Tommy gun and hits a wide range of systems.
I do want to warn squeamish readers about another issue: The novel can be quite graphic at times, explicitly detailing the intimacy struggles with the main couple. It borders on the pornographic side, but not for titillation. It’s meant to show the dysfunction in the supposedly happy couple.
There were quite a few humorous moments, such as the protagonist enjoying the sounds of the chickens, only to grow wistful about their impending deaths so she can taste chicken broth. Or the protagonist’s evangelical mother reacting to her son-in-law’s purchase of a yoga mat.
I was a little disappointed in the resolution of the central issue, i.e., the time travel. But overall, the novel was an enjoyable read and worth your time.
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Reviewed in the United States on May 8, 2026